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We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells