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A specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophileThiobacillus caldus and the thermophilic archaeonSulfolobus acidocaldarius was developed. It employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes.

An Immunological Assay for Detection and Enumeration of Thermophilic Biomining Microorganisms