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Seven monoclonal antibodies (MAbs) toErwinia carotovora subsp.atroseptica have been produced. One, called 4G4, reacted with high specificity for serogroup I ofE. carotovora subsp.atroseptica , the most common serogroup on potato tubers in different serological assays. Eighty-six strains belonging to differentE. carotovora subsp.atroseptica serogroups were assayed. Some strains of serogroup XXII also reacted positively. No cross-reactions were observed against other species of plant pathogenic bacteria or 162 saprophytic bacteria from potato tubers. Only one strain ofE. chrysanthemi from potato cross-reacted. A comparison of several serological techniques to detectE. carotovora subsp.atroseptica on potato tubers was performed with MAb 4G4 or polyclonal antibodies. The organism was extracted directly from potato peels of artificially inoculated tubers by soaking or selective enrichment under anaerobiosis in a medium with polypectate. MAb 4G4 was able to detect specifically 240E. carotovora subsp.atroseptica cells per ml by indirect immunofluorescence and immunofluorescence colony staining and after soaking by ELISA-DAS (double-antibody sandwich enzyme-linked immunosorbent assay) after enrichment. The same amount of cells was detected by using immunolectrotransfer with polyclonal antibodies, andE. carotovora subsp.atroseptica and subsp.carotovora were distinguished by the latter technique. ELISA-DAS using MAb 4G4 with an enrichment step also efficiently detectedE. carotovora subsp.atroseptica in naturally infected tubers and plants.

applied and environmental microbiology

Characterization of Monoclonal Antibodies Specific for <i>Erwinia carotovora</i> subsp. <i>atroseptica</i> and Comparison of Serological Methods for Its Sensitive Detection on Potato Tubers

Characterization of Monoclonal Antibodies Specific for Erwinia carotovora subsp. atroseptica and Comparison of Serological Methods for Its Sensitive Detection on Potato Tubers