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The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthesis of azoverdin, under both nitrogen-fixing and ammonium-assimilating conditions, was observed.

Nitrogen Assimilation: Optimization of 15 N-Labelled Azoverdin Production by Azomonas macrocytogenes ATCC 12334

Nitrogen Assimilation: Optimization of ¹⁵ N-Labelled Azoverdin Production by Azomonas macrocytogenes ATCC 12334