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The triacylglycerol (TG) profiles of teliospores ofTilletia controversa andTilletia tritici were examined by high-performance liquid chromatography (HPLC) and gas-liquid chromatography. Boiling isopropanol was used to ensure enzyme inactivation during homogenization. The largest lipid component as determined by thin-layer chromatography was TGs. On the basis of thin-layer chromatography of crude lipid extracts,T. controversa andT. tritici do not contain a large amount of free fatty acids. TG profiles ofT. controversa andT. tritici were very similar, with 18 species of TGs resolved by HPLC and gas-liquid chromatography. In both organisms, PLL (palmitic, linoleic, linoleic) was the major component, followed by LLL (trilinolein) and PLO (palmitic, linoleic, oleic). The ratio of PLO to PLL was 1:6 and 1:4 inT. tritici andT. controversa , respectively. The TGs of both organisms contain long-chain (&gt;22 carbons) mono- and dienoic acids. Linoleic acid was the major fatty acid found in TGs from both organisms. The differences of TGs were not considered significant for differentiation purposes.

Triacylglycerol Profiles of Tilletia controversa and Tilletia tritici