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The incorporation of [3 H]thymidine by axenic cultures of anaerobic bacteria was investigated as a means to measure growth. The three fermentative strains and one of the methanogenic strains tested incorporated [3 H]thymidine, whereas the sulfate-reducing bacterium and two of the methanogenic bacteria were unable to incorporate [3 H]thymidine during growth. It is concluded that the [3 H]thymidine incorporation method underestimates bacterial growth in anaerobic environments.

[ 3 H]Thymidine Incorporation To Estimate Growth Rates of Anaerobic Bacterial Strains