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A biological indicator based on fluorimetric detection within 60 min of aBacillus stearothermophilus spore-bound enzyme, α-d -glucosidase, has been developed. Results indicate that the enzyme survived slightly longer than spores observed after 24 h of incubation. The new system shows promise for evaluating flash sterilization cycles within 60 min compared with conventional 24-h systems.

applied and environmental microbiology

Fluorimetric Detection of a <i>Bacillus stearothermophilus</i> Spore-Bound Enzyme, α- <scp>d</scp> -Glucosidase, for Rapid Indication of Flash Sterilization Failure

Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, α- d -Glucosidase, for Rapid Indication of Flash Sterilization Failure