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A region of DNA which determined the production of the insecticidal toxin of Bacillus thuringiensis subsp. israelensis was cloned into a derivative of a broad-host-range group IncQ plasmid vector of gram-negative bacteria. The plasmid which we constructed was transferred by conjugative mobilization into a Bradyrhizobium species that nodulates pigeon peas. In this species the construction was maintained stably in the absence of selection and expressed the gene that was installed. Experiments in a greenhouse with the strain which we constructed indicated that this organism provides protection against root nodule damage by the larvae of the insect Rivellia angulata (Diptera).

applied and environmental microbiology

Limiting an Insect Infestation of Nitrogen-Fixing Root Nodules of the Pigeon Pea ( <i>Cajanus cajan</i> ) by Engineering the Expression of an Entomocidal Gene in Its Root Nodules

Limiting an Insect Infestation of Nitrogen-Fixing Root Nodules of the Pigeon Pea ( Cajanus cajan ) by Engineering the Expression of an Entomocidal Gene in Its Root Nodules