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A selective medium was developed for the dilution plate isolation ofMycoleptodiscus terrestris from natural soils and sediments from aquatic environments. The ingredients per liter of the selective medium are as follows: KH2 PO4 , 0.5 g; MgSO4 7H2 O, 0.5 g; dextrose, 10.0 g; peptone, 5.0 g; chloramphenicol, 0.25 g; rose bengal, 50 mg; oxgall, 5.0 g; Terraclor (pentachloronitrobenzene, 75% active ingredients), 0.5 g; agar, 15.0 g. After autoclaving, the following ingredients were aseptically added: sorbic acid (0.7% autoclave-sterilized aqueous solution), 5.0 ml; Subdue (25.1% emulsion of metalaxyl), 0.5 ml; Truban (40.7% suspension of etridiazol), 0.05 ml. The colony-restrictive properties of this medium enabled its use in the drop plate method, originally developed for viable counts of bacteria. Alfalfa sprouts as baits were not suitable for quantitative recovery of the fungus, although 5% of alfalfa sprouts were infected withM. terrestris when incubated on soil containing 1.5 × 102 CFU/g.

Selective Medium for Isolation of Mycoleptodiscus terrestris from Soil Sediments of Aquatic Environments