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An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be stored at −85°C without loss of competence. WithLactococcus lactis subsp.cremoris BC101, a transformation frequency of 5.7 × 107 transformants per μg of pIL253 DNA was obtained, which represents 5% of the surviving cells. All the lactococcal strains tested could be transformed by the present method.

applied and environmental microbiology

High-Frequency Transformation, by Electroporation, of <i>Lactococcus lactis</i> subsp. <i>cremoris</i> Grown with Glycine in Osmotically Stabilized Media

High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media