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Aseptic isolation of the facultative gut microflora of the tobacco hornworm,Manduca sexta , yielded four microorganisms. Two were gram-positiveBacillus spp., one wasSerratia plymuthica , and another was the yeastCandida guilliermondii . The three bacterial species were screened for extrachromosomal DNA, andS. plymuthica was found to have a 6.4-kilobase plasmid, which was designated pCP-1.

Isolation of Plasmid-Harboring Serratia plymuthica from Facultative Gut Microflora of the Tobacco Hornworm, Manduca sexta