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The membrane phospholipid fatty acids (PLFAs) from several cocultures and a pure culture ofSyntrophomonas wolfei were determined by capillary column gas chromatography. Cocultures ofS. wolfei with aDesulfovibrio sp. contained PLFAs from both organisms, whereas PLFAs from a coculture withMethanospirillum hungatei contained very little biomass to analyze. The pure culture ofS. wolfei grown on crotonate provided the best material for analysis of the PLFAs. The predominant PLFAs ofS. wolfei were the monounsaturated 16:1ω7c and 16:1ω9c and the saturated 16:0 and 14:0. A low concentration of the diunsaturated 18:2ω6 was detected. The PLFA analysis provides additional information for consideration in the determination of the profile of PLFAs obtained from anaerobic environments. In addition, this information may aid in the understanding of the physiology and phylogeny ofS. wolfei and other syntrophic bacteria.

Phospholipid Fatty Acid Composition of the Syntrophic Anaerobic Bacterium Syntrophomonas wolfei