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With both chymotrypsin and mutanolysin used to form protoplasts, consistent transformation frequencies of 104 to 105 transformants and transfectants per μg of DNA were achieved. The procedure was used to transform protoplasts ofStreptococcus cremoris CS224 at low frequency (5 transformants per μg of DNA).Images

Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts