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Acremonium coenophialum , an endophytic fungus present in toxic tall fescue grass and seed, grew very slowly or not at all with conventional media and cultural practices. However, a considerable increase in growth was achieved in a relatively dilute medium consisting solely of glucose and yeast extract. The optimal levels of glucose and yeast extract were 3 to 6% and 0.35% (wt/vol), respectively. The addition of salts which lowered the pH suppressed growth. Even when the pH was controlled, the addition of KH2 PO4 at a level of 3.2% or more greatly inhibited growth.A. coenophialum grew better in shake culture than in stationary culture. The optimal temperature was 23°C, and the optimal pH was 6.5.

In Vitro Growth of Acremonium coenophialum , an Endophyte of Toxic Tall Fescue Grass