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The ability to ferment the trisaccharide raffinose was linked with the presence of plasmid DNA in three strains ofPediococcus pentosaceus. Parental strains showed associated inducible α-galactosidase and sucrose hydrolase activities when grown in α-galactosides and sucrose, respectively. Derivative strains of PPE1.0, PPE2.0, and PPE5.0, which had lost 30-, 28-, and 23-megadalton plasmids, respectively, had no α-galactosidase or sucrose hydrolase activity.

Evidence for Plasmid Linkage of Raffinose Utilization and Associated α-Galactosidase and Sucrose Hydrolase Activity in Pediococcus pentosaceus