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The organism Streptomyces chartreusis IMRU 3962 produced a mixture of the antibiotics hydroheptin and chartreusin in fermentation broth. The addition of increasing levels of phosphate resulted in a corresponding increase in the production of both antibiotics, with maximum yields of 400 to 450 micrograms of chartreusin per ml and 80 to 100 micrograms of hydroheptin per ml at 0.45 to 0.55 M phosphate. Chartreusin was invariably produced at a higher ratio; however, a reversal in ratio to favor hydroheptin was attained when 0.03% copper sulfate was added to the medium, particularly at a 0.2 M KH2PO4 level, with antibiotic yields of 125 micrograms of hydroheptin per ml and 40 micrograms of chartreusin per ml.

Effect of phosphate and copper on the fermentation of hydroheptin