Open AccessOnly part of the protoxin gene of Bacillus thuringiensis subsp. berliner 1715 is necessary for insecticidal activity
Author(s) -
Hiroetsu Wabiko,
Gary A. Held,
Lee A. Bulla
Publication year - 1985
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.49.3.706-708.1985
Subject(s) - bacillus thuringiensis , manduca sexta , biology , gene , mutant , escherichia coli , manduca , microbiology and biotechnology , larva , bacteria , genetics , botany
Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicating that only a part of the protoxin gene is required for insecticidal activity.
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