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Laboratory studies of methane formation in peat samples from an acid subarctic mire in Sweden indicated the presence of a low-temperature-adapted methanogenic flora. Enrichment culture studies with ethanol, acetate, hydrogen, or a combination of these as substrate for methane formation provided evidence for the existence of two different methanogenic populations in the peat: one, unaffected by hydrogen and using acetate, with a temperature optimum at 20°C; the other, oxidizing hydrogen, with a temperature optimum at ca. 28°C.

Different Temperature Optima for Methane Formation When Enrichments from Acid Peat Are Supplemented with Acetate or Hydrogen