Open AccessProduction of Antimicrobial and Bacteriocin-Like Substances by Rhizobium trifolii
Author(s) -
M. V. Joseph,
J. D. Desai,
Anjana J. Desai
Publication year - 1983
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.45.2.532-535.1983
Subject(s) - lysozyme , antimicrobial , strain (injury) , trypsin , microbiology and biotechnology , bacteriocin , agar plate , rhizobium , biology , agar , food science , bacteria , chemistry , biochemistry , enzyme , anatomy , genetics
Rhizobium trifolii strains IARI and Rel-1 produced substances with broad and narrow activity spectra, respectively. Reproducible inhibitory zones of various sizes produced by R. trifolii IARI (2 to 14 mm) and R. trifolii Rel-1 (2 to 6 mm) were detected, depending upon the indicator organism used. The maximum production of these substances by both strains of R. trifolii was observed on l-arabinose agar. A preliminary characterization of the antimicrobial substance produced by strain IARI showed resistance to heat (75 to 80 degrees C for 45 min), trypsin, lysozyme, DNase I, and RNase A. On the other hand, the substance produced by strain Rel-1 showed sensitivity to heat (75 to 80 degrees C for 45 min) and trypsin, but resistance to lysozyme, RNase A, and DNase I.
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