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Growing cultures ofPachysolen tannophilus concurrently consumed and produced ethanol in the presence of substantial concentrations ofd -xylose. Ethanol was also assimilated in the presence of other sugars, the amount depending on the sugar. Less ethanol assimilation occurred withd -glucose than withd -xylose. The rate of ethanol consumption decreased as the concentration of glucose was increased, but some consumption still occurred when 2% glucose was present. The rate increased with the amount of oxygen available to the culture whend -xylose or ethanol was the carbon source. In most instances, estimates of consumption were based on the extent of incorporation of14 C from [1-14 C]ethanol into trichloroacetic acid-insoluble material. The results are pertinent to the use ofP. tannophilus for the production of ethanol fromd -xylose.

Concurrent Production and Consumption of Ethanol by Cultures of Pachysolen tannophilus Growing on d -Xylose