Enhanced Production of Surfactin from Bacillus subtilis by Continuous Product Removal and Metal Cation Additions

The lipopeptide, surfactin, is produced byBacillus subtilis. A study has been made on large-scale production of this surfactant. A good yield was obtained from a glucose substrate fermentation by continuously removing the product by foam fractionation. The surfactin could be easily recovered from the collapsed foam by acid precipitation. The yield was also improved by the addition of either iron or manganese salts. Hydrocarbon addition to the medium, which normally increases biosurfactant production, completely inhibited surfactin production byB. subtilis.