Open AccessRegio-Selective 10-Hydroxylation of Patchoulol, a Sesquiterpene, by Pithomyces Species
Author(s) -
Yasuji Suhara,
Sayuri Itoh,
Mayumi Ogawa,
Kazuteru Yokose,
Toyoaki Sawada,
Takashi Sano,
Rieko Ninomiya,
Hiromi Maruyama
Publication year - 1981
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.42.2.187-191.1981
Subject(s) - sesquiterpene , fermentation , hydroxylation , biology , chemistry , botany , food science , biochemistry , enzyme
Of some 350 microorganisms screened, four strains ofPithomyces species were found to carry out regio-selective hydroxylation of patchoulol, a sesquiterpene, to 10-hydroxypatchoulol:Pithomyces sp. NRJ201,P. chartarum NRJ210, and, to a lesser extent,P. cynodontis ATCC 26150 andP. atro-olivaceus IFO 6651 were found to catalyze this reaction. A method has been developed by which 10-hydroxypatchoulol was obtained in 25 to 45% yields in 1- to 5-liter fermentation jars at 2 to 4 g of patchoulol per liter and isolated as pure material in 30% yields.
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