Open AccessExtraction of rotavirus from human feces by treatment with lithium dodecyl sulfate
Author(s) -
M. C. Croxson,
A.R. Bellamy
Publication year - 1981
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.41.1.255-260.1981
Subject(s) - feces , rotavirus , sodium dodecyl sulfate , chromatography , chemistry , gel electrophoresis , virus , virology , polyacrylamide gel electrophoresis , human feces , isolation (microbiology) , lithium (medication) , microbiology and biotechnology , biology , biochemistry , enzyme , endocrinology
A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.
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