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Proportions of respiring bacteria determined with a 2-(p -iodophenyl)-3-(p -nitrophenyl)-5-phenyl tetrazolium chloride dye-epifluorescent technique were significantly elevated in the 300-μm surface layer of a salt marsh estuary. Almost all the detectably respiring bacteria in the particle-laden surface layer and a significant proportion in subsurface waters were attached to particles.

Enumeration of Particle-Bound and Unattached Respiring Bacteria in the Salt Marsh Environment