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A biological process for converting naphthalene tocis -1,2-dihydroxy-1,2-dihydronaphthalene (DHD) catalyzed byPseudomonas putida strain 119 was optimized in flask experiments. These studies revealed the following: (i)P. putida 119 can propagate efficiently and produce DHD when supplied one of several carbon sources and naphthalene; (ii) maximum DHD production byP. putida 119 occurs in logarithmic-growth-phase cells and decreases at various rates in the stationary growth phase, depending upon the carbon source used; (iii) several analogs of salicylic acid can be used as effective inducers of naphthalene metabolism inP. putida cells growing on glucose; and (iv) the addition of chemical surfactants to naphthalene-cell (P. putida 119) mixtures stimulates DHD production.

Biological Process for Converting Naphthalene to cis -1,2-Dihydroxy-1,2-Dihydronaphthalene