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Sphaerotilus natans grew at the maximum specific growth rate (μmax ) of 0.43/h when cultivated on PGY medium at 25°C. The organism mainly grew attached to inside of the culture vessels when the culture medium was fed to the completely mixed continuous-flow apparatus at a dilution rate above μmax and the attached growth was directly related to the dilution rate. When a low concentration of the medium was supplied to the apparatus, almost all of the cells grown were filamentous and attached to the inside of the vessels. When a high concentration of the medium was fed, the organism grew as single cells or short chains and flowed out into the effluent. The attached growth ofS. natans in the continuous-flow apparatus was inhibited by the minimal inhibitory concentration of 0.5 to 1.0 μg of 9-β-d -arabinofuranosyladenine per ml. 9-β-d -Arabinofuranosyladenine showed bacteriocidal activity againstS. natans at a concentration of 50 to 100 μg/ml.

Attached Growth of Sphaerotilus natans in Continuous-Flow Apparatus and Its Growth Inhibition by 9-β- d -Arabinofuranosyladenine