Open AccessMethod for Adenosine 5′-Triphosphate Measurement on Coke Waste Activated Sludge
Author(s) -
James R. Russell,
Joseph Gauthier
Publication year - 1978
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.35.2.450-452.1978
Subject(s) - activated sludge , adenosine triphosphate , chemistry , coke , extraction (chemistry) , luciferin , phenol , sodium dodecyl sulfate , adenosine , chromatography , biochemistry , waste management , luciferase , pulp and paper industry , wastewater , organic chemistry , transfection , engineering , gene
Measurement of adenosine 5′-triphosphate (ATP) in coke waste activated sludge can provide a simple method for estimating the levels of viable microbes in the sludge. However, the presence of inhibitors such as phenol in the sludge interferes when the luciferin-luciferase method is used to measure ATP. These inhibiting substances can be removed from the sludge before extraction of ATP by washing the cells with dilute sodium dodecyl sulfate.
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