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Thiobacillus thiooxidans increases the acid formation from certain types of the pyritic concretions in coal known as sulfur balls, as well as from marcasite. Thiobacillus ferrooxidans increases the acid formed from museum grade pyrite of fine particle size as well as from sulfur balls. A general mechanism for acid formation in coal mines is proposed.

Autotropic Bacteria and the Formation of Acid in Bituminous Coal Mines