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Previous studies implicated loss of motility and mutations of thealsR andsigW regulatory genes in enhanced fitness of theBacillus subtilis evolved strain WN716 over that of its ancestral strain WN624. The fitness of strains carrying knockout mutationsalsR ::spc ,sigD ::kan , and/orsigW ::erm was measured and compared to that of the congenic ancestral strain by competition experiments.

Increased Competitive Fitness of Bacillus subtilis under Nonsporulating Conditions via Inactivation of Pleiotropic Regulators AlsR, SigD, and SigW