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The recombinant industrial Saccharomyces cerevisiae strain MA-R5 was engineered to express NADP(+)-dependent xylitol dehydrogenase using the flocculent yeast strain IR-2, which has high xylulose-fermenting ability, and both xylose consumption and ethanol production remarkably increased. Furthermore, the MA-R5 strain produced the highest ethanol yield (0.48 g/g) from nonsulfuric acid hydrolysate of wood chips.

applied and environmental microbiology

Efficient Bioethanol Production by a Recombinant Flocculent <i>Saccharomyces cerevisiae</i> Strain with a Genome-Integrated NADP <sup>+</sup> -Dependent Xylitol Dehydrogenase Gene

Efficient Bioethanol Production by a Recombinant Flocculent Saccharomyces cerevisiae Strain with a Genome-Integrated NADP + -Dependent Xylitol Dehydrogenase Gene

Efficient Bioethanol Production by a Recombinant Flocculent Saccharomyces cerevisiae Strain with a Genome-Integrated NADP ⁺ -Dependent Xylitol Dehydrogenase Gene