English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Water quality monitoring techniques that target microorganisms in the orderBacteroidales are potential alternatives to conventional methods for detection of fecal indicator bacteria.Bacteroidales and members of the genusBacteroides have been the focus of microbial source tracking (MST) investigations for discriminating sources of fecal pollution (e.g., human or cattle feces) in environmental waters. For accurate source apportionment to occur, one needs to understand both the abundance ofBacteroides in host feces and the survival of these host-associated microbial markers after deposition in the environment. Studies were undertaken to evaluate the abundance, persistence, and potential for growth ofBacteroidales originating from poultry litter under oxic and anoxic environmental conditions.Bacteroidales abundance, as determined by quantitative PCR (qPCR) with GenBac primers and probe, increased 2 to 5 log gene copies ml−1 and 2 log gene copies g litter−1 under most conditions during incubation of poultry litter in a variety of laboratory microcosm and field mesocosm studies. DNA sequencing of theBacteroidales organisms in the litter identified taxa with sequences corresponding exactly to the GenBac primer and probe sequences and that were closely related toBacteroides uniformis ,B. ovatus , andB. vulgatus . These results suggest that MST studies using qPCR methods targetingBacteroidales in watersheds that are affected by poultry litter should be interpreted cautiously. Growth ofBacteroidales originating from poultry litter in environmental waters may occur whileBacteroidales growth from other fecal sources declines, thus confounding the interpretation of MST results.

Evidence for Extraintestinal Growth of Bacteroidales Originating from Poultry Litter