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Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.

applied and environmental microbiology

Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1 , Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288