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In this study,Pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain twoiaaL paralogs, which are both chromosomally located in most strains. Comparative analysis ofiaaL nucleotide sequences amplified from these two paralogs revealed that one paralog,iaaL Psn , is 100% identical toiaaL fromP. savastanoi pv. nerii, while the other paralog,iaaL Psv , exhibited 93% identity toiaaL fromPseudomonas syringae pv. tomato (iaaL Pto ). A 3-nucleotide motif (TAC) comprised of 3 to 15 repeats, which remained stable after propagation of the strains in olive plants, was found iniaaL Psv . Based on the observed nucleotide sequence variations, a restriction fragment length polymorphism assay was developed that allowed differentiation amongiaaL Psn ,iaaL Psv , andiaaL Pto . In addition, reverse transcriptase PCR on total RNA fromP. savastanoi pv. savastanoi strains demonstrated that bothiaaL Psv andiaaL Psn containing 14 or fewer TAC repeats are transcribed. Capillary electrophoresis analysis of PCR-amplified DNA fragments containing the TAC repeats fromiaaL Psv allowed the differentiation ofP. savastanoi pv. savastanoi isolates.

applied and environmental microbiology

<i>Pseudomonas savastanoi</i> pv. savastanoi Contains Two <i>iaaL</i> Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat

Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat