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A 16S rRNA-targeted, Cy3-labeled oligonucleotide probe was designed to detect members of the genus Bdellovibrio by fluorescence in situ hybridization. Specific hybridization conditions were established; however, the detection of bdellovibrios in environmental samples required enrichment, confirming that Bdellovibrio spp. are not present in large numbers in the environment.

applied and environmental microbiology

Design and Performance of a 16S rRNA-Targeted Oligonucleotide Probe for Detection of Members of the Genus <i>Bdellovibrio</i> by Fluorescence In Situ Hybridization

Design and Performance of a 16S rRNA-Targeted Oligonucleotide Probe for Detection of Members of the Genus Bdellovibrio by Fluorescence In Situ Hybridization