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A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones I, II, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.

applied and environmental microbiology

Multiplex PCR for Simultaneous Detection of Bacteria of the Genus Listeria , Listeria monocytogenes , and Major Serotypes and Epidemic Clones of L. monocytogenes