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To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.

applied and environmental microbiology

Revisiting with a Relative-Density Calibration Approach the Determination of Growth Rates of Microorganisms by Use of Optical Density Data from Liquid Cultures