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Melissococcus plutonius is a fastidious honeybee pathogen, and the addition of KH2 PO4 to culture medium is required for its growth. Using genome sequences and a newly developed vector, we showed that mutations in genes encoding Na+ /H+ antiporter and cation-transporting ATPase are involved in the potassium requirement for growth.

Identification of Mutations Involved in the Requirement of Potassium for Growth of Typical Melissococcus plutonius Strains