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Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter.

applied and environmental microbiology

New Insights into the <i>Enterococcus faecalis</i> CroRS Two-Component System Obtained Using a Differential-Display Random Arbitrarily Primed PCR Approach

New Insights into the Enterococcus faecalis CroRS Two-Component System Obtained Using a Differential-Display Random Arbitrarily Primed PCR Approach