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In this study, we tested the hypothesis that the SdiA proteins ofEscherichia coli andSalmonella enterica serovar Typhimurium respond to indole. While indole was found to have effects on gene expression and biofilm formation, these effects were notsdiA dependent. However, high concentrations of indole did inhibitN -acyl-l -homoserine lactone (AHL) sensing by SdiA. We conclude that SdiA does not respond to indole but indole can inhibit SdiA activity inE. coli andSalmonella .

The Acyl Homoserine Lactone Receptor, SdiA, of Escherichia coli and Salmonella enterica Serovar Typhimurium Does Not Respond to Indole