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Iron, a major protein cofactor, is essential for most organisms but can simultaneously be toxic. Iron homeostasis thus has to be effectively maintained under a range of iron regimes. This may be particularly true withShewanella oneidensis , a representative of dissimilatory metal-reducing bacteria (DMRB), which are capable of respiring a variety of chemicals as electron acceptors (EAs), including iron ores. Although iron respiration and its regulation have been extensively studied in this bacterium, how iron homeostasis is maintained remains largely unknown. Here, we report that the loss of the iron homeostasis master regulator Fur negatively affects the respiration of all EAs tested. This defect appears mainly to be a result of reduced cytochromec (cytc ) production, despite a decrease in the expression of reductases that are under the direct control of Fur. We also show thatS. oneidensis Fur interacts with canonical Fur box motifs in F-F-x-R configuration rather than the palindromic motif proposed before. Thefur mutant has lowered total iron and increased free iron contents. Under iron-rich conditions, overproduction of the major iron storage protein Bfr elevates the total iron levels of thefur mutant over those of the wild-type but does not affect free iron levels. Intriguingly, such an operation only marginally improves cytc production by affecting hemeb biosynthesis. It is established that iron dictates hemeb /cytc biosynthesis inS. oneidensis fur + strains, but thefur mutation annuls the dependence of hemeb /cytc biosynthesis on iron. Overall, our results suggest that Fur has a profound impact on the iron homeostasis ofS. oneidensis , through which many physiological processes, especially respiration, are transformed.IMPORTANCE Iron reduction is a signature ofS. oneidensis , and this process relies on a large number of typec cytochromes, whichper se are iron-containing proteins. Thus, iron plays an essential and special role in iron respiration, but to date, the nature of iron metabolism and regulation of the bacterium remains largely unknown. In this study, we investigated impacts of Fur, the master regulator of iron homeostasis, on respiration. The loss of Fur causes a general defect in respiration, a result of impaired cytc production rather than specific regulation. Additionally, thefur mutant is unresponsive to iron, resulting in imbalanced iron homeostasis and dissociation between iron and cytc production. These findings provide important insights into the iron biology of DMRB.

Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants