Open AccessThe aadB Gene Cassette Is Associated with bla SHV Genes in Klebsiella Species Producing Extended-Spectrum β-Lactamases
Author(s) -
Louisa A. Jones,
Christopher J. McIver,
MiJurng Kim,
William D. Rawlinson,
Peter A. White
Publication year - 2005
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.49.2.794-797.2005
Subject(s) - integron , gene cassette , biology , plasmid , amplicon , gene , microbiology and biotechnology , enterobacteriaceae , klebsiella pneumoniae , genetics , polymerase chain reaction , escherichia coli
Integrons were detected in 37 (72.5%) of 51 Klebsiella spp. producing extended-spectrum beta-lactamases by PCR with primers that targeted integrase genes and cassette regions. PCR and amplicon sequencing of the cassette regions revealed aadB and aadA2 gene cassettes that confer resistance to a range of aminoglycosides. aadB was associated with a class 1 integron on a 28-kb plasmid, pES1, that also contained bla(SHV-12) and IS26.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom