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The LightCycler was compared to nested PCR for the detection of bla(GES/IBC) genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla(GES/IBC) gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.

antimicrobial agents and chemotherapy

Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from <i>Pseudomonas aeruginosa</i> by Use of a Real-Time PCR Assay

Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay