Open AccessDevelopment of a Yeast Assay for Rapid Screening of Inhibitors of Human-Derived Pneumocystis carinii Dihydrofolate Reductase
Author(s) -
Liang Ma,
Qiuyao Jia,
Joseph A. Kovacs
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.9.3101-3103.2002
Subject(s) - dihydrofolate reductase , pneumocystis carinii , complementation , biology , saccharomyces cerevisiae , protein fragment complementation assay , yeast , enzyme , strain (injury) , virology , microbiology and biotechnology , biochemistry , pneumocystis jirovecii , gene , phenotype , human immunodeficiency virus (hiv) , anatomy
Human-derived Pneumocystis carinii dihydrofolate reductase (DHFR) was expressed in a Saccharomyces cerevisiae strain whose growth depends on complementation by this enzyme. We utilized a quantitative assay to measure the sensitivity of this yeast strain to DHFR inhibitors. This assay should be useful for identifying new inhibitors of human-derived P. carinii DHFR.
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