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Using an isogenic system, we have determined the impact that the four promoters known to control bla(TEM) gene expression have on beta-lactamase activity. For both TEM-1 and TEM-30, this activity gradually increased in relation to the presence of promoters P3, Pa/Pb, and P4 upstream of the corresponding gene. Promoter P5, only found upstream of the bla(TEM-1B) gene, was related to the highest expression of this gene.

antimicrobial agents and chemotherapy

Promoters P3 , Pa / Pb , P4 , and P5 Upstream from bla TEM Genes and Their Relationship to β-Lactam Resistance