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Rifampin is an antibiotic that interacts specifically with the β-subunit of DNA-dependent RNA polymerase (RpoB) encoded by the rpoB gene ([2][1]). Rifampin resistance (Rifr) is usually due to changes in the amino acid sequence of the target site resulting in reduced affinity of RpoB for rifampin.

Natural Rifampin Resistance in Treponema spp. Correlates with Presence of N531 in RpoB Rif Cluster I