Open AccessBiochemical-Genetic Characterization and Distribution of OXA-22, a Chromosomal and Inducible Class D β-Lactamase fromRalstonia(Pseudomonas)pickettii
Author(s) -
Patrice Nordmann,
Laurent Poirel,
Matthew Kubina,
Anne Casetta,
Thierry Naas
Publication year - 2000
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.44.8.2201-2204.2000
Subject(s) - biology , cloxacillin , gene , microbiology and biotechnology , ralstonia , genetics , cephalosporin , genomic dna , pseudomonadaceae , pseudomonas , bacteria , antibiotics , staphylococcus aureus
From genomic DNA of Ralstonia pickettii isolate PIC-1, a beta-lactamase gene was cloned that encodes the oxacillinase OXA-22. It differs from known oxacillinases, being most closely related to OXA-9 (38% amino acid identity). The hydrolytic spectrum of OXA-22 is limited mostly to benzylpenicillin, cloxacillin, and restricted-spectrum cephalosporins. OXA-22-like genes were identified as single chromosomal copies in five other R. pickettii clinical isolates. The expression of OXA-22-like beta-lactamases was inducible in R. pickettii.
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