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An enzyme-linked immunosorbent assay was performed directly on fixed, virus-infected cell monolayers to rapidly determine the susceptibility of herpes simplex virus to antiviral drugs. Susceptibility determinations by this method correlated well with those obtained by plaque reduction assay and avoided some of the drawbacks of other methods currently available.

Rapid herpes simplex virus susceptibility testing using an enzyme-linked immunosorbent assay performed in situ on fixed virus-infected monolayers