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Escapin is anl -amino acid oxidase that acts on lysine to produce hydrogen peroxide (H2 O2 ), ammonia, and equilibrium mixtures of several organic acids collectively called escapin intermediate products (EIP). Previous work showed that the combination of synthetic EIP and H2 O2 functions synergistically as an antimicrobial toward diverse planktonic bacteria. We initiated the present study to investigate how the combination of EIP and H2 O2 affected bacterial biofilms, usingPseudomonas aeruginosa as a model. Specifically, we examined concentrations of EIP and H2 O2 that inhibited biofilm formation or fostered disruption of established biofilms. High-throughput assays of biofilm formation using microtiter plates and crystal violet staining showed a significant effect from pairing EIP and H2 O2 , resulting in inhibition of biofilm formation relative to biofilm formation in untreated controls or with EIP or H2 O2 alone. Similarly, flow cell analysis and confocal laser scanning microscopy revealed that the EIP and H2 O2 combination reduced the biomass of established biofilms relative to that of the controls. Area layer analysis of biofilms posttreatment indicated that disruption of biomass occurs down to the substratum. Only nanomolar to micromolar concentrations of EIP and H2 O2 were required to impact biofilm formation or disruption, and these concentrations are significantly lower than those causing bactericidal effects on planktonic bacteria. Micromolar concentrations of EIP and H2 O2 combined enhancedP. aeruginosa swimming motility compared to the effect of either EIP or H2 O2 alone. Collectively, our results suggest that the combination of EIP and H2 O2 may affect biofilms by interfering with bacterial attachment and destabilizing the biofilm matrix.

Inhibition and Dispersal of Pseudomonas aeruginosa Biofilms by Combination Treatment with Escapin Intermediate Products and Hydrogen Peroxide