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New Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistantEscherichia coli isolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lowerk cat /Km ratios for all the β-lactams tested except for doripenem. The genetic context forbla NDM-3 wastnpA-bla NDM-3 -ble MBL -trpF-dsbC-tnpA-sulI-qacEdeltaI-aadA2-dfrA1 , which was present on an approximately 250-kb plasmid.

Biochemical Analysis of Metallo-β-Lactamase NDM-3 from a Multidrug-Resistant Escherichia coli Strain Isolated in Japan