Open AccessInteraction of the Echinocandin Caspofungin with Amphotericin B or Voriconazole against Aspergillus Biofilms In Vitro
Author(s) -
Weixia Liu,
Lijuan Li,
Yi Sun,
Wei Chen,
Zhe Wan,
Ruoyu Li,
Wei Liu
Publication year - 2012
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00687-12
Subject(s) - caspofungin , voriconazole , echinocandin , microbiology and biotechnology , amphotericin b , echinocandins , aspergillus , antifungal , biofilm , in vitro , biology , medicine , bacteria , biochemistry , fluconazole , genetics
Aspergillus biofilms were prepared from 22 strains ofAspergillus spp. via a 96-well plate-based method. Using a broth microdilution checkerboard technique with the XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] colorimetric assay, we demonstrated a synergistic antifungal activity against 18 of 22Aspergillus biofilm strains with a combination of caspofungin and amphotericin B and against 13 of 22 strains with a combination of caspofungin and voriconazole. We did not observe antagonism.
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